Description
Short Description: Recombinant SETMAR (accession number NP_006506.3) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 79 kDa. SETMAR (SET Domain and mariner transposase fusion gene) is a ubiquitously expressed nuclear fusion protein that contains an N-terminal SET domain with histone methyltransferase activity and a C-terminal mariner (MAR) transposase domain that recognizes and binds DNA. The gene exists as a fusion gene only in anthropoid primates; other organisms lack mariner transposase domain. SETMAR binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The mariner transposase domain recognizes and binds the 19-mer core of the 5'-TIR (terminal inverted repeats) of the Hsmar1 element. The SET domain specifically methylates lysines 4 and 36 of histone H3 which are epigenetic marks associated with transcriptional activation. SETMAR demonstrates in vivo end joining activity and may mediate genomic integration of foreign DNA. Recombinant SETMAR can be used to study enzyme kinetics and perform inhibitor screening assays